The interaction between acetylcholine and carbachol, and frusemide, a loop diuretic, have been studied on the rat isolated urinary bladder strip preparation. Acetylcholine (4.36 x 10(-8) – 1.3 x 10(-6) M) and carbachol (5.5 x 10(-8) – 6.9 x 10(-6) M) induced contractions and these were significantly potentiated by frusemide (3.02 x 10(-6) M). The ratio of EC50 in the absence of frusemide to EC50 in the presence of frusemide was 1.58 +/- 0.03 (s.e.m.) for acetylcholine and 1.86 +/- 0.14 for carbachol. Potentiation of acetylcholine and carbachol contractions by frusemide was not observed in tissues treated with hexamethonium (2.5 x 10(-5) M). Rhythmic contractions induced by frusemide alone were markedly reduced by hexamethonium (2.5 x 10(-5) M) and tetrodotoxin (10(-6) M) but they were not significantly reduced by atropine (1.7 x 10(-6) M). The result suggests that frusemide increases the sensitivity of the bladder to acetylcholine and carbachol, and that it may have a nicotinic stimulant effect on the bladder. This extra-renal action may contribute to its prompt diuretic property.
Journal of Pharmacy and Pharmacology 09/1990; 42(8):597-8. DOI:10.1111/j.2042-7158.1990.tb07070.x