The bactericidal activity of 10 and 5% ethanol alone and in the presence of EDTA against ps. aeruginosa, E. coli and Staph. aureus has been studied using the extinction-time and the death-curve methods. EDTA at various concentrations was found to potentiate the bactericidal activity of ethanol. This potentiation increased with increase in the EDTA concentration from 0.05 to 0.3% against Ps. aeruginosa and Staph. aureus. Similar results were obtained with E. coli in EDTA concentrations of 0.05–0.2% but not with 0.3% which showed the least potentiating effect.
International Journal of Pharmaceutics 12/1983; 17(2-3-17):121-128. DOI:10.1016/0378-5173(83)90025-X