Ultraviolet absorption spectroscopy was used to establish the wavelength of maximum absorbance for pure powder of artesunate and then the Beer’s plot generated. This was validated and used to assay nine brands (X1-X9) of artesunate in Nigerian drug market.
Distinctive ultraviolet absorption at 287 nm of pure sample of Artesunate in simulated intestinal fluid (SIF) afforded a simple, precise and the most reliable method for the analysis of nine different brands of Artesunate marketed in Nigeria. SIF does not have any appreciable absorption in the ultraviolet region. This simple method yielded a Beer’s plot for Artesunate with high correlation (R2) of 0.9972 +/- 0.00016 and was reproducible. The Beer’s plot was obeyed in concentration range of 10-200 mg%. The limits of detection (sensitivity) and quantitation were found to be 0.471 mg/ml and 1.27 mg/ml respectively. The results showed that only four out of the nine brands assayed had deviations from label claims that were within acceptable limits.
Based on these convincing data, simple ultraviolet spectroscopy at 287 nm could be used to assay artesunate in formulations.
Evidence for the spectroscopic determination of Artesunate in dosage form (PDF Download Available). Available from: https://www.researchgate.net/publication/24143323_Evidence_for_the_spectroscopic_determination_of_Artesunate_in_dosage_form [accessed Dec 26, 2015].