The influence of time of poultry manure application: no application, at planting, 1, 2 and 3 months after planting (MAP) were determined on the growth, yield and fruit quality of two plantain (Musa spp. AAB) cultivars (PITA 17 and French Reversion). PITA 17 significantly produced more standing leaves, fruits with longer green and shelf life. French Reversion significantly produced taller plants, heavier bunches and fruits, wider and longer fruits. Dry matter partitioning to the pulp, pulp degree of lightness and redness were also higher for French Reversion than PITA 17. The application of poultry manure at 2 MAP produced heaviest bunches and the highest yield components while pulp dry matter and fruit shelf life duration were highest with manure application at 3 MAP. Bunch and fruit weights were heaviest in French Reversion plots applied with poultry manure at 2 MAP whereas shelf life was longest with PITA 17 that received manure application at planting.